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biotinylated mouse anti-human ifn-γ monoclonal antibody  (Mabtech Inc)

 
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    Structured Review

    Mabtech Inc biotinylated mouse anti-human ifn-γ monoclonal antibody
    Biotinylated Mouse Anti Human Ifn γ Monoclonal Antibody, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-human+ifn-%CE%B3+monoclonal+antibody/anti+ifn+%CE%B3+antibody/pmc11542980-214-16-23
    Average 90 stars, based on 1 article reviews
    biotinylated mouse anti-human ifn-γ monoclonal antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Enzyme-linked Immunospot:

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 μg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: Risk Factors and Immune Response to Hepatitis E viral Infection among Acute Hepatitis Patients in Assiut, Egypt
    Article Snippet: Briefly, 96 well plates with polyvinylidene difluoride membrane (Millipore MAIPS 4510) were coated with mouse anti-human IFN-γ monoclonal antibody (MAbTech clone 7-B6-1) at a concentration of 10μg/ml of phosphate buffered saline (PBS).

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: IFN-γ Enzyme-Linked Immunospot (ELISPOT) Assay.

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 pg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: SARS-CoV-2 XBB.1.5 mRNA Booster Vaccination Elicits Limited Mucosal Immunity
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from Mabtech at 1 μg per well and incubated overnight at 4°C.

    Article Title: Study of the BCG Vaccine-Induced Cellular Immune Response in Schoolchildren in Antananarivo, Madagascar
    Article Snippet: Briefly, PBMC were thawed and counted, then placed in 96-well plates with well membranes of polyvinylidene fluoride (PVDF Millipore): the plates had already been coated for 2 hours with 100μl/well of mouse anti-human IFN-γ monoclonal antibody (mAb 1-D1K, cat: 3420-3-1000, MabTech) at 10 μg/ml, washed in phosphate-buffered saline (PBS), and blocked with R10 medium.

    Article Title: Phylogenetic analysis consistent with a clinical history of sexual transmission of HIV-1 from a single donor reveals transmission of highly distinct variants
    Article Snippet: 100 μl of 0.5 μ g/ml mouse anti-human IFN-γ monoclonal antibody solution (Mabtech, Sweden) was added to each well on an ELISpot plate (Millipore, US).

    Article Title: Fragmentation of SIV-gag Vaccine Induces Broader T Cell Responses
    Article Snippet: Plates were washed 3 times with sterile PBS and coated overnight with mouse anti-human IFN-γ monoclonal antibody (10 μg/ml, clone 1-D1K, Mabtech AB, Sweden) in sterile PBS.

    Incubation:

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 μg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: Risk Factors and Immune Response to Hepatitis E viral Infection among Acute Hepatitis Patients in Assiut, Egypt
    Article Snippet: Briefly, 96 well plates with polyvinylidene difluoride membrane (Millipore MAIPS 4510) were coated with mouse anti-human IFN-γ monoclonal antibody (MAbTech clone 7-B6-1) at a concentration of 10μg/ml of phosphate buffered saline (PBS).

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: IFN-γ Enzyme-Linked Immunospot (ELISPOT) Assay.

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 pg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: SARS-CoV-2 XBB.1.5 mRNA Booster Vaccination Elicits Limited Mucosal Immunity
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from Mabtech at 1 μg per well and incubated overnight at 4°C.

    Article Title: Study of the BCG Vaccine-Induced Cellular Immune Response in Schoolchildren in Antananarivo, Madagascar
    Article Snippet: Briefly, PBMC were thawed and counted, then placed in 96-well plates with well membranes of polyvinylidene fluoride (PVDF Millipore): the plates had already been coated for 2 hours with 100μl/well of mouse anti-human IFN-γ monoclonal antibody (mAb 1-D1K, cat: 3420-3-1000, MabTech) at 10 μg/ml, washed in phosphate-buffered saline (PBS), and blocked with R10 medium.

    Article Title: Phylogenetic analysis consistent with a clinical history of sexual transmission of HIV-1 from a single donor reveals transmission of highly distinct variants
    Article Snippet: 100 μl of 0.5 μ g/ml mouse anti-human IFN-γ monoclonal antibody solution (Mabtech, Sweden) was added to each well on an ELISpot plate (Millipore, US).

    Article Title: Fragmentation of SIV-gag Vaccine Induces Broader T Cell Responses
    Article Snippet: Plates were washed 3 times with sterile PBS and coated overnight with mouse anti-human IFN-γ monoclonal antibody (10 μg/ml, clone 1-D1K, Mabtech AB, Sweden) in sterile PBS.

    Concentration Assay:

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 μg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: Risk Factors and Immune Response to Hepatitis E viral Infection among Acute Hepatitis Patients in Assiut, Egypt
    Article Snippet: Briefly, 96 well plates with polyvinylidene difluoride membrane (Millipore MAIPS 4510) were coated with mouse anti-human IFN-γ monoclonal antibody (MAbTech clone 7-B6-1) at a concentration of 10μg/ml of phosphate buffered saline (PBS).

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: IFN-γ Enzyme-Linked Immunospot (ELISPOT) Assay.

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 pg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: SARS-CoV-2 XBB.1.5 mRNA Booster Vaccination Elicits Limited Mucosal Immunity
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from Mabtech at 1 μg per well and incubated overnight at 4°C.

    Article Title: Study of the BCG Vaccine-Induced Cellular Immune Response in Schoolchildren in Antananarivo, Madagascar
    Article Snippet: Briefly, PBMC were thawed and counted, then placed in 96-well plates with well membranes of polyvinylidene fluoride (PVDF Millipore): the plates had already been coated for 2 hours with 100μl/well of mouse anti-human IFN-γ monoclonal antibody (mAb 1-D1K, cat: 3420-3-1000, MabTech) at 10 μg/ml, washed in phosphate-buffered saline (PBS), and blocked with R10 medium.

    Article Title: Phylogenetic analysis consistent with a clinical history of sexual transmission of HIV-1 from a single donor reveals transmission of highly distinct variants
    Article Snippet: 100 μl of 0.5 μ g/ml mouse anti-human IFN-γ monoclonal antibody solution (Mabtech, Sweden) was added to each well on an ELISpot plate (Millipore, US).

    Article Title: Fragmentation of SIV-gag Vaccine Induces Broader T Cell Responses
    Article Snippet: Plates were washed 3 times with sterile PBS and coated overnight with mouse anti-human IFN-γ monoclonal antibody (10 μg/ml, clone 1-D1K, Mabtech AB, Sweden) in sterile PBS.

    Sequencing:

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 μg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: Risk Factors and Immune Response to Hepatitis E viral Infection among Acute Hepatitis Patients in Assiut, Egypt
    Article Snippet: Briefly, 96 well plates with polyvinylidene difluoride membrane (Millipore MAIPS 4510) were coated with mouse anti-human IFN-γ monoclonal antibody (MAbTech clone 7-B6-1) at a concentration of 10μg/ml of phosphate buffered saline (PBS).

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: IFN-γ Enzyme-Linked Immunospot (ELISPOT) Assay.

    Article Title: Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from MabTech at a concentration of 1 pg/well overnight at 4° C. Plates were washed with DPBS, and blocked with R10 media (RPMI with 10% heat inactivated FBS with 1% of 100× penicillin-streptomycin, 1M HEPES, 100 mM Sodium pyruvate, 200 mM L-glutamine, and 0.1% of 55 mM 2-Mercaptoethanol) for 2-4 h at 37° C. The Spike 1 and Spike 2 peptide pools contain 15 amino acid peptides overlapping by 11 amino acids that span the protein sequence and reflect the N- and C-terminal halves of the protein, respectively.

    Article Title: SARS-CoV-2 XBB.1.5 mRNA Booster Vaccination Elicits Limited Mucosal Immunity
    Article Snippet: ELISPOT plates were coated with mouse anti-human IFN-γ monoclonal antibody from Mabtech at 1 μg per well and incubated overnight at 4°C.

    Article Title: Study of the BCG Vaccine-Induced Cellular Immune Response in Schoolchildren in Antananarivo, Madagascar
    Article Snippet: Briefly, PBMC were thawed and counted, then placed in 96-well plates with well membranes of polyvinylidene fluoride (PVDF Millipore): the plates had already been coated for 2 hours with 100μl/well of mouse anti-human IFN-γ monoclonal antibody (mAb 1-D1K, cat: 3420-3-1000, MabTech) at 10 μg/ml, washed in phosphate-buffered saline (PBS), and blocked with R10 medium.

    Article Title: Phylogenetic analysis consistent with a clinical history of sexual transmission of HIV-1 from a single donor reveals transmission of highly distinct variants
    Article Snippet: 100 μl of 0.5 μ g/ml mouse anti-human IFN-γ monoclonal antibody solution (Mabtech, Sweden) was added to each well on an ELISpot plate (Millipore, US).

    Article Title: Fragmentation of SIV-gag Vaccine Induces Broader T Cell Responses
    Article Snippet: Plates were washed 3 times with sterile PBS and coated overnight with mouse anti-human IFN-γ monoclonal antibody (10 μg/ml, clone 1-D1K, Mabtech AB, Sweden) in sterile PBS.



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    Image Search Results


    (a) ELISA-on-a-chip designed for LCD 3D printing with structurally encoded sequential delivery of reagents to autonomously perform an assay and coupled with a built-in chip-to-assay connection. (b) ELISA workflow showing the sandwich immunoassay designed for the detection of IFN-γ by sequentially delivering assay reagents and wash buffer to a nitrocellulose membrane pre-spotted with anti-human IFN-γ capture antibody; (1) sample containing IFN-γ, (2) biotinylated anti-human IFN-γ detection antibody, (3) streptavidin-conjugated enzyme pHRP, and (4) enzyme substrate in the presence of hydrogen peroxide to generate the colorimetric readout. (c) Autonomous CC workflow for on-chip reagent aliquoting by metering the correct reagent volumes and drainage of the excess, followed by (d) MCR-based sequential delivery of assay reagents with wash steps in between. Arrows show the direction of flow. Scale bar = 5 mm. (e) Binding curve of the on-chip assay for the detection of IFN-γ with a limit of detection of 12 pg mL -1 (CV: 6.8%) across triplicate chips for each tested concentration point; line shows a 4-point logistic fit.

    Journal: bioRxiv

    Article Title: High-resolution low-cost LCD 3D printing of microfluidics

    doi: 10.1101/2023.12.31.573772

    Figure Lengend Snippet: (a) ELISA-on-a-chip designed for LCD 3D printing with structurally encoded sequential delivery of reagents to autonomously perform an assay and coupled with a built-in chip-to-assay connection. (b) ELISA workflow showing the sandwich immunoassay designed for the detection of IFN-γ by sequentially delivering assay reagents and wash buffer to a nitrocellulose membrane pre-spotted with anti-human IFN-γ capture antibody; (1) sample containing IFN-γ, (2) biotinylated anti-human IFN-γ detection antibody, (3) streptavidin-conjugated enzyme pHRP, and (4) enzyme substrate in the presence of hydrogen peroxide to generate the colorimetric readout. (c) Autonomous CC workflow for on-chip reagent aliquoting by metering the correct reagent volumes and drainage of the excess, followed by (d) MCR-based sequential delivery of assay reagents with wash steps in between. Arrows show the direction of flow. Scale bar = 5 mm. (e) Binding curve of the on-chip assay for the detection of IFN-γ with a limit of detection of 12 pg mL -1 (CV: 6.8%) across triplicate chips for each tested concentration point; line shows a 4-point logistic fit.

    Article Snippet: Purified mouse monoclonal IgG anti-human interferon-γ capture antibody (Cat. #MAB2852, lot #FIO1022021, R&D Systems, Minneapolis, Minnesota, United States), biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat. #285-IF, lot #RAX2422031, R&D Systems, Minneapolis, Minnesota, United States), Pierce streptavidin poly-horseradish peroxidase (pHRP) (Cat. #21140, lot #XJ360080, Thermo Fisher Scientific, Waltham, Massachusetts, United States), SIGMAFAST 3,3’-Diaminobenzidine tablets (Cat. #D4293, lot #SLCG5357, Sigma-Aldrich, Oakville, Ontario, Canada), bovine serum albumin (BSA) (Cat. #001-000-162, lot #162191, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, United States), BSA-biotin (Cat. #A8549, Sigma-Aldrich, Oakville, Ontario, Canada), Tween 20 (Cat. #P7949, lot #SLBX0835, Sigma-Aldrich, Oakville, Ontario, Canada).

    Techniques: Enzyme-linked Immunosorbent Assay, Membrane, Binding Assay, Concentration Assay